João Vasco Ferreira

Investigador  Investigador Doutorado
Principal Investigator

João Ferreira is a biologist and Principal Investigator at NOVA Medical School, with an MSc in Molecular and Cellular Biology from the University of Coimbra and a joint PhD from Albert Einstein College of Medicine and the University of Coimbra. His research focuses on extracellular vesicle biology, hypoxia signaling, and autophagy, with a strong interest in translating mechanistic discoveries into therapeutic innovation.

João identified HIF1A as a substrate of chaperone-mediated autophagy and later helped define a LAMP2A/HSC70-dependent mechanism for selective protein loading into exosomes. This work led to the discovery of ExoSignal, a platform for engineering exosome cargo loading.

He has secured competitive funding as PI, including FCT projects, established his independent laboratory in 2025, and currently lead a multidisciplinary team developing exosome-based strategies for intracellular delivery in vascular and retinal disease.

  • Ferreira JV, Ferraz LC, da Rosa Soares A, Ejtehadifar M, Carvalho AS, Hall MJ, Morello J, Beck HC, Ramalho JS, Matthiesen R, Pereira P. LAMP2A regulates endosomal protein composition and membrane identity in exosome biogenesis. iScience. 2025 Dec 2;29(1):114305. doi: 10.1016/j.isci.2025.114305. PMID: 41503212; PMCID: PMC12768870.
  • Carvalho Ferraz L, Pereira P, Ferreira JV. Molecular Mechanisms of Extracellular Vesicle Biogenesis and Their Impact on the Design of Custom EVs. Adv Healthc Mater. 2025 Sep;14(23):e2501349. doi: 10.1002/adhm.202501349. Epub 2025 Jul 8. PMID: 40624965; PMCID: PMC12417789.
  • Ferreira JV, da Rosa Soares A, Pereira P. LAMP2A mediates the loading of proteins into endosomes and selects exosomal cargo. Autophagy. 2022 Sep;18(9):2263-2265. doi: 10.1080/15548627.2022.2092315. Epub 2022 Jun 27. PMID: 35761480; PMCID: PMC9397471.
  • Ferreira JV, da Rosa Soares A, Pereira P. Cell Non-autonomous Proteostasis Regulation in Aging and Disease. Front Neurosci. 2022 Jun 9;16:878296. doi: 10.3389/fnins.2022.878296. PMID: 35757551; PMCID: PMC9220288.
  • Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P. LAMP2A regulates the loading of proteins into exosomes. Sci Adv. 2022 Mar 25;8(12):eabm1140. doi: 10.1126/sciadv.abm1140. Epub 2022 Mar 25. PMID: 35333565; PMCID: PMC8956266.
  • Ferreira JV, Rosa Soares A, Ramalho JS, Ribeiro-Rodrigues T, Máximo C, Zuzarte M, Girão H, Pereira P. Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis. PLoS One. 2019 Oct 15;14(10):e0223790. doi: 10.1371/journal.pone.0223790. PMID: 31613922; PMCID: PMC6794069.
  • Soares AR, Martins-Marques T, Ribeiro-Rodrigues T, Ferreira JV, Catarino S, Pinho MJ, Zuzarte M, Isabel Anjo S, Manadas B, P G Sluijter J, Pereira P, Girao H. Gap junctional protein Cx43 is involved in the communication between extracellular vesicles and mammalian cells. Sci Rep. 2015 Aug 19;5:13243. doi: 10.1038/srep13243. Erratum in: Sci Rep. 2015 Oct 08;5:14888. doi: 10.1038/srep14888. PMID: 26285688; PMCID: PMC4541155.
  • Ferreira JV, Soares AR, Ramalho JS, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015 May 11;5:10210. doi: 10.1038/srep10210. PMID: 25958982; PMCID: PMC4426689.
  • Ferreira JV, Fôfo H, Bejarano E, Bento CF, Ramalho JS, Girão H, Pereira P. STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy. Autophagy. 2013 Sep;9(9):1349-66. doi: 10.4161/auto.25190. Epub 2013 Jun 7. PMID: 23880665.
  • Ferreira JV. Diabetes, hypoxia and cardiovascular disease: From molecular mechanism to treatment. Rev Port Cardiol. 2017 May;36(5):375-376. English, Portuguese. doi: 10.1016/j.repc.2017.03.003. Epub 2017 May 10. PMID: 28499719.
  • Medal of Honor Decoration for Outstanding Achievement, Municipality of Chaves, Portugal 2022
  • Assistant Researcher Contract, NOVA University of Lisboa, Portugal 2023-now
  • PostDoc Fellowship Awarded by FCT, Portugal (SFRH/BPD/121271/2016) 2017-now
  • Assistant Researcher Contract, University of Coimbra, Portugal 2015-2017
  • PhD Fellowship Awarded by FCT 2009-2012
  • SCOPUS identifier 55858420400
  • ORCID 0000-0001-5897-7461
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