Plataforma de Biologia Molecular

A Plataforma de Biologia Molecular oferece à Comunidade Científica acesso a um excelente serviço personalizado de biologia molecular. Esta plataforma implementou um processo simplificado que é capaz de gerar uma grande variedade de construções de DNA, virais e não virais, de forma rápida, eficiente e económica. Também oferece produção de proteína recombinante.

Serviço de Biologia Molecular preço

Subcloning cDNA/DNA PCR cloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Cloning (≤ 500 bp) 150-300€
DNA Cloning (501 bp -1.5 kb) 200-500€
DNA Cloning (≥1.5 kb) 40€/h

Subcloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Subcloning (≤2,000 bp) 200-300€
DNA Subcloning (>2,000 bp) 40€/h

Site-directed mutagenesis
Site-directed mutagenesis (1-2 reactions/construct) 300-400€
Site-directed mutagenesis (3-5 reactions/construct) 400-500€

cDNA/DNA PCR cloning (Gateway®)
DNA PCR cloning into pENTR (≤2,000 bp) (eg pCR8) 200-300€
DNA PCR cloning into pENTR (>2,000 bp) 40€/h
Subcloning into prokaryotic or mammalian vectors, several tags available
Subcloning into lentiviral vectors (vectors available pLenti6, pLenti6/CMV) 200-300€
Subcloning into adenoviral vectors (vectors available pAd, pAd/CMV) 300-500€

Virus production
Lentivirus production 300-400€
Adenovirus production 200-500€

Protein production (prokaryotic system) 500-1000€
Protein production (eukaryotic system) 40€/h

Other services available

(all reagents/materials and consumables are included)

Contactar o Coordernador por email/telefone

Coordenador Científico

José Ramalho
Researcher

Coordenador Executivo

José Ramalho
Researcher

Equipa

Carin Didoss
Sergio Muñoz Cobos
Hakan Onur