Microscopy Facility

The Microscopy Facility possesses the scientific know-how and technical knowledge in advanced microscopy and image analysis to support and enhance scientific research. We also provide access to a wide variety of instruments covering from basic to advanced light microscopy, available to all research community (from NOVA Medical School and external users).

The Facility staff assists researchers in the experimental planning of light microscopy experiments, provides in-depth training for the operation of the microscopes as well as troubleshooting during microscopes operation. Additionally, support is provided in the processing, rendering and analysis of acquired datasets. If needed, custom analysis routines will be provided as a service.

For the continued high-quality service, it is our mission to keep our resources updated and our staff on top of the latest microscopy developments and techniques.

EQUIPMENT

  • Confocal Zeiss LSM 980 with Airyscan 2
  • Confocal Zeiss LSM 710
  • Confocal Andor Spinning disk
  • Widefield Observer Z1 for HCS
  • Widefield Axioimager Z2
  • Stereoscope Lumar V12 with fluorescence
  • Stereoscope Leica MZ16A with fluorescence
  • Stereoscope Stereo V8 with fluorescence
  • Multiple smaller Stereoscopes
  • Workstation 1 with Imaris 10.2, Huygens 24.10, ZEN 3.1 Workstation
  • Confocal Zeiss cell observer spinning disk
  • Multiple smaller Widefield Microscopes with/without Fluorescence

SERVICES

  • Training in all microscopes, for independent usage
  • Consulting and initial scientific project/experiment planning
  • Consulting in sample preparation
  • Training in image processing software usage (Fiji, IMARIS, Icy,..)
  • Image processing and analysis services: Workflow automation (macros, scripting);
  • Image processing Workflow consulting and development.
  • Image processing custom software development
  • Consultation for a publication

Microscopy Facility usage is open to all community. Users must register on the equipment booking platform (Agendo) and request equipment permissions before using the systems. Alternatively for external users we can be contacted via email. Proper training is always required to use the facility resources in self service mode.

  • Portuguese Platform of Bio Imaging (PPBI)
  • Euro Bioimaging (EuBi)
  • Lisbon BioImaging Group (LxBIG)
  • PT-OPENSCREEN
  • EU-OPENSCREEN
  • BioData.pt
  • CTLS-Core Tecnologies for Live Sciences
  • QUAREP-LiMi

Facility Manager

Telmo Pereira
Microscopy Facility Manager