Cell Culture Facility

EQUIPMENTS

• Room dedicated to cell line culture
  This room is equipped with HEPA filters, four laminar flow chambers, and eight incubators, one of which is designated for quarantine, ensuring the highest level of quality control.
• Room dedicated to the cultivation of human and animal primary cells
  The space's organization and specialized equipment are designed to minimize any risk of cross-contamination, enabling researchers to work efficiently and safely with primary cells.
• Room dedicated to the culture of stem cells and certified primary human cells
  One of our highlights in this room is the Neon Transfection System, which offers high precision in the transfection of DNA, RNA, or CRISPR/Cas9 into challenging cells. Is in this room that organoids are studied, offering advanced possibilities for investigating complex cellular models.
• Room exclusively dedicated to working with viruses
  This room is designed to provide a controlled and safe environment, equipped with all the necessary tools for the secure handling of viruses.

SERVICES

• Development and Validation of Advanced 3D Models (Organoids and Tumoroids) for Biomarker Discovery and Validation
  The Cell Culture Facility serves as an "In Vitro 3D Models Unit" to work with organoids derived from primary animal cultures, organoids and tumoroids from primary human cultures, and organoids differentiated from human induced pluripotent stem cells (iPS cells). These models offer a more physiologically relevant environment than traditional 2D cultures and are crucial for identifying and validating biomarkers associated with environmental factors and immune responses. The Cell Culture facility has already developed 3D skin models for melanoma and retinal models for diabetic retinopathy, both with high translational potential.
• Personalized 3D Bioprinting Services for Disease Modeling
  The presence of a bioprinter at the Cell Culture Facility enables the creation of more complex and reproducible 3D tissue models, including single- or multi-organ-on-a-chip platforms. This service can offer the design and bioprint specific tissue architectures, allowing the research of complex interactions between environmental factors and human health, and the innovative discovery of relevant biomarkers.
• Preclinical Validation of Biomarkers in Regulatory-Compliant 3D Models
  Through the HubP2C, that aims to accelerate technological maturation towards TRL 5–6 and is strongly committed to Quality and Regulatory Compliance, we implement the best practices in our biomarker validation services using 3D models highly innovative. Under strict quality and regulatory standards, we bridge the gap between basic research and clinical application.

The Cell Culture Facilty also provides trainning and consultancy to all the facility's users.

CTLS - Core Technologies for Life Sciences