CEDOC - Biologia Molecular R Camara Pestana 6 1150-082 Lisboa
Telefone: 218803000 Ext: 26090

Jose da Silva Ramalho

Investigador Investigador Principal
Principal researcher at Molecular Biology platform that provides research support to furthering the research projects within CEDOC and partners worldwide. With his expertise in animal and cell models of diseases as well as expertise in transgenic and gene editing provides support in Molecular Biology techniques from basic to more complex cloning/gene editing with high capabilities in numerous cloning strategies and access to a suite of vectors that includes adenovirus and lentivirus. José Ramalho is involved in a range of collaboration/services combining advice/training and access to cutting-edge techniques to meet gene analysis needs. In addition, the platform in collaboration with individual researchers produces, specific recombinant proteins for a wide range of applications, including structural and functional studies, small ligand screening and antibody production used in scientific projects.
Robinson CL, Evans RD, Briggs DA, Ramalho JS, Hume AN. Deficiency in kinesin-1 recruitment to melanosomes precludes it from facilitating their centrifugal transport. J Cell Sci. 2017 May. PMID:28490438 Correia MS, Moreiras H, Pereira FJC, Neto MV, Festas TC, Tarafder AK, Ramalho JS, Seabra MC, Barral DC. Melanin Transferred to Keratinocytes Resides in Nondegradative Endocytic Compartments. J Invest Dermatol. 2017 Oct. PMID:29074272 Carrascal MA, Silva M, Ramalho JS, Pen C, Martins M, Pascoal C, Amaral C, Serrano I, Oliveira MJ, Sackstein R, Videira PA. Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation and ERK1/2 and p38 MAPK activation. Mol Oncol. 2017 Dec. PMID: 29215790 Calado J, Santos AR, Aires I, Lebre F, Nolasco F, Rueff J, Ramalho J. The Na+ -coupled glucose transporter SGLT2 interacts with its accessory unit MAP17 in vitro and their expressions overlap in the renal proximal tubule. FEBS Lett. 2018 Oct. PMID:30156268 Bover O, Justo T, Pereira PNG, Facucho-Oliveira J, Inácio JM, Ramalho JS, Domian IJ, Belo JA. Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells. PLoS One. 2018. PMID:30281646 Robinson CL, Evans RD, Sivarasa K, Ramalho JS, Briggs DA, Hume AN. The adaptor protein melanophilin regulates dynamic myosin-Va:cargo interaction and dendrite development in melanocytes. Mol Biol Cell. 2019 Jan. PMID:30699046 Casal D, Iria I, Ramalho JS, Alves S, Mota-Silva E, Mascarenhas-Lemos L, Pontinha C, Guadalupe-Cabral M, Ferreira-Silva J, Ferraz-Oliveira M, Vassilenko V, Goyri-O'Neill J, Pais D, Videira PA. BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection. Sci Rep. 2019 May. PMID:31133641 Kuhns S, Seixas C, Pestana S, Tavares B, Nogueira R, Jacinto R, Ramalho JS, Simpson JC, Andersen JS, Echard A, Lopes SS, Barral DC, Blacque OE. Rab35 controls cilium length, function and membrane composition. EMBO Rep. 2019 Oct. PMID:31432619 Casalou C, Faustino A, Silva F, Ferreira IC, Vaqueirinho D, Ferreira A, Castanheira P, Barona T, Ramalho JS, Serpa J, Félix A, Barral DC. Arl13b Regulates Breast Cancer Cell Migration and Invasion by Controlling Integrin-Mediated Signaling. Cancers (Basel). 2019 Sep. PMID:31569511 Ferreira JV, Rosa Soares A, Ramalho JS, Ribeiro-Rodrigues T, Máximo C, Zuzarte M, Girão H, Pereira P. Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis. PLoS One. 2019 Oct. PMID:31613922 Catarino S, Ribeiro-Rodrigues TM, Sá Ferreira R, Ramalho J, Abert C, Martens S, Girão H. A Conserved LIR Motif in Connexins Mediates Ubiquitin-Independent Binding to LC3/GABARAP Proteins. Cells. 2020 Apr. PMID: 32272685 Lopes R, Shi K, Fonseca NA, Gama A, Ramalho JS, Almeida L, Moura V, Simões S, Tidor B, Moreira JN. Modelling the impact of nucleolin expression level on the activity of F3 peptide-targeted pH-sensitive pegylated liposomes containing doxorubicin. Drug Deliv Transl Res. 2021 Apr. PMID: 33860446 Marques IJ, Gomes I, Pojo M, Pires C, Moura MM, Cabrera R, Santos C, van IJcken WFJ, Teixeira M, Ramalho JS, Leite V, Cavaco BM. Identification of SPRY4 as a novel candidate susceptibility gene for familial non-medullary thyroid cancer. Thyroid. 2021 Apr. PMID: 33906393 Cristina Escrevente C, Bento-Lopes L, Ramalho JS, Barral DC. Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB. J Cell Sci. 2021 May. PMID: 34002205 Alves LS, Marques ARA, Padrão N, Carvalho FA, Ramalho J, Lopes C, Soares MIL, Futter CE, Pinho E Melo TMVD, Santos NC, Vieira OV. J Cell Sci. 2021 Sep. PMID: 34528688
ORCID: 0000-0002-1927-164X Ciencia Vitae: FE13-BC0C-D253 Scopus Author ID: 7003401533 ResearcherID: M-6415-2015