Neurogenetics of Locomotion

Our research aims to understand the mechanisms, genes and circuits that govern coordinated locomotion under normal and anomalous or pathological conditions.

Behaviorally, we intend to understand how the ensemble of sensory circuits, central pattern generators, motor neurons and higher order centers collectively interact in order to optimize locomotion under different settings, including pathological conditions.

For this we use the genetically tractable model system Drosophila melanogaster, which combines powerful genetic tools with sophisticated and detailed kinematic quantification approaches. Considering how motor systems and the genes that define them are evolutionarily conserved, this work will impact the study of motor control of more complex organisms and contribute to the study of human motor conditions. Moreover, the tools and approaches we use can easily be translated into more complex organism including rodents and humans. 

Graphical Abstract

"A Genetic model for neurorehabilitation” PTDC/BIA-COM/0151/2020, Foundation for Science and Technology (FCT), Principal investigator

“Senescent cells and their secretory phenotype: novel targets for spinal cord repair” -MC-36-2020 Prémio Melo e Castro 2020, Santa Casa da Misericórdia de Lisboa; Partener (Principal investigator: Leonor Saúde, iMM)

"Development of an inexpensive, simple and open-source device for SARS-Cov-2 detection." Projetos de implementação rápida para soluções inovadoras - COVID-19, #613788604; Foundation for Science and Technology (FCT). Principal investigator

“Testing the protective effect of candidate compounds in a Drosophila model of Amyotrophic Lateral  Sclerosis (ALS)”, iNOVA4Health, UNL; Co- Principal investigator (with Claudia Santos, ITQB)

"A Genetic model for neurorehabilitation" H2020-MSCA-IF-2016, #752891 Marie Skłodowska-Curie program –Reintegration Grant-; European Commission. Principal investigator

“Neurogenetics of Locomotion” Investigator FCT; IF/01154/2014/CP1252/CT0003, Foundation for Science and Technology (FCT), Principal investigator

  • A Cabrita, AM Medeiros, T Pereira, AS Rodrigues, M Kranendonk, CS Mendes, (2022) “Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity” 15 Jul 2022, In: ISCIENCE. 25, 7, p. 104541; bioRxiv 2021.07.09.451676. 
  • Sardoo A, Neto A, Torres RP, Rodrigues-Manica S, Domingues L, Crespo CL, Lagoas-Gomes J, Mascarenhas V, Mendes CS, Galzerano A, Almeida SFd, Sepriano A, Ramiro S, Masi A, Nair K, Costa J, Alexandre B, Vassilevskaia T, Cunha C, Sobral D, Branco J, Gomes-Alves P, Pimentel-Santos F. (2021) “The role of muscle in the susceptibility and progression of axial Spondyloarthritis: The MyoSpA Study Protocol”, Acta Reumatol Port. Oct-Dec 2021;46(4):342-349. 
  • AR Fernandes, CS Mendes, ER Gomes, RO Teodoro, (2021) “Motor neuron boutons remodel through membrane blebbing” bioRxiv 2021.03.07.434250 
  • F Heredia, Y Volonté, J Pereirinha, M Fernandez-Acosta, AP Casimiro, CG Belém, F Viegas, K Tanaka, J Menezes, M Arana, G A. Cardoso, A Macedo, M Kotowicz, F H. Prado Spalm, MJ Dibo, RD Monfardini, TT Torres, CS Mendes, A Garelli, AM Gontijo (2021) “Innate Behavior Sequence Progression by Peptide-Mediated Interorgan Crosstalk” Nature Communications, 07 June 2021 
  • S Skarlatou,; C Hérent; E Toscano; CS Mendes; J Bouvier; N Zampieri. "Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection". Cell Reports 31 10 (2020): 107741. 
  • CS Mendes*, I Bartos, Z Márka, T Akay, S Márka, RS Mann*. (2015) “Quantification of gait parameters in freely walking rodents”. BMC Biology, 22 July, 13:50. * corresponding authors. 
  • MB Bouchard, V Voleti, CS Mendes, C Lacefield, WB Grueber, RS Mann, RM Bruno and EMC Hillman, (2015) “Swept Light Sheet (SLS) imaging for ultra-fast, volumetric microscopy in awake, behaving organisms”, Nature Photonics, Feb;9(2):113-119 (cover article). 
  • CS Mendes*, SV Rajendren, I Bartos, S Márka, RS Mann*. (2014) “Kinematic responses to changes in walking orientation and gravitational load in Drosophila melanogaster”, PLoS ONE 9(10): e109204. * corresponding authors.  
  • CS Mendes, I Bartos, T Akay, S Márka, RS Mann. (2013) “Quantification of gait parameters in freely walking wild type and sensory deprived Drosophila melanogaster”. eLife, January 8. 
  • CS Mendes, C Levet , G Chatelain, P Dourlen, A Fouillet, ML Dichtel-Danjoy, A Gambis, HD Ryoo, H Steller, B Mollereau. (2009) “ER stress protects from retinal degeneration.” The EMBO Journal ;28(9):1296-307. 
  • CS Mendes, E Arama, S Brown, H Scherr, M Srivastava, A Bergmann, H Steller, B Mollereau (2006) “Cytochrome-c-d regulates developmental apoptosis in the Drosophila retina” EMBO reports ;7(9):933-9 (cover article). 
  • RD Almeida, BJ Manadas, CV Melo, JR Gomes, CS Mendes, MM Grãos, RF Carvalho, AP Carvalho and CB Duarte (2005) “Neuroprotection by BDNF against glutamate-induced apoptotic cell death is mediated by ERK and PI3-kinase pathways” Cell Death and Differentiation 12: 1329–1343 
  • PM Domingos, M Mlodzik, CS Mendes, S Brown, H Steller, B Mollereau (2004) “Spalt transcription factors are required for R3/R4 specification and establishment of planar cell polarity in the Drosophila eye. ” Development. 131(22):5695-702. 

2021, Best poster, Portuguese Drosophila meeting (to Anna Hobbiss)
2020, Best talk, Portuguese Drosophila meeting (to Anna Hobbiss)
2019, Best Basic Research Talk - iMed Innovate Competition (to Ricardo Custódio)
2017, Marie Sklodowska-Curie program, Reintegration Grant
2014, FCT investigator, Starting Grant
2014, Drosophila Image Award, runner up, Genetics Society of America
2011, Travel grant, XII Portuguese Society for Neuroscience Meeting

2 PhD Research Opportunities @ Neurogenética da Locomoção Lab
Contract for Auxililary Investigator @ Neurogenetics of Locomotion Lab

Pursuant to the provisions of article 11, paragraph 2, of Decree-Law no. 57/2016, of August 29, it becomes public that by Dean’s decree of February 10, 2022, the opening of an international selection competition procedure was authorized for the completion of a Assistant Investigator job vacancy for the exercise of scientific research activities financed by the Fundação para a Ciência e Tecnologia, under an uncertain fixed-term employment contract under the Labor Code in the Faculdade de Ciências Médicas|NOVA Medical School, Universidade NOVA de Lisboa.

Application Deadline: March 25th 2022

  • Alisson Gontijo, CEDOC 
  • Cláudia Santos, CEDOC 
  • Fernando Pimentel-Santos, CEDOC 
  • Hugo Miranda, CEDOC 
  • Leonor Saúde, iMM 
  • Marta Moita, CCU 
  • Michel Kranendonk, ToxOmics 
  • Niccolo Zampieri, Max-Delbrück-Centrum for Molecular Medicine, Germany 
  • Rita Teodoro, CEDOC 
  • Sandra Tenreiro, CEDOC 
  • Sebastião Rodrigues, ToxOmics 
  • Vasco Barreto, CEDOC 

Principal Investigator

César Mendes


Anna Hobbiss
Post-Doc Researcher
Alexandra Medeiros
PhD Student
Inês Fernandes
MSc Student
Manuel Tanqueiro
MSc Student